A role for microglia in the maintenance of photoreceptors in retinal transplants lacking pigment epithelium

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Abstract
Studies on intact retina have pointed to a necessary role for retinal pigment epithelium in the maintenance of photoreceptor outer segments and for regeneration of visual pigment. However, it has been shown that when embryonic retinae are separated from the pigment epithelium and transplanted into the brain of neonatal rats, the transplanted photoreceptors develop outer segments and the retina responds to light in the apparent absence of pigment epithelial cells. We confirm that there are no retinal pigment epithelium cells associated with transplanted retinae in the present series of experiments and show that a row of cells, composed predominantly of microglia of host origin, border the graft. These cells can be seen to contain engulfed outer segments when they are apposed to the outer retina, suggesting that the microglia have assumed, at the least, the phagocytic function normally associated with retinal pigment epithelium. Microglial cells and their processes are also found within the transplant, but these cells are typically devoid of phagosornes, indicating an absence of phagocytic activity. The close physical association of these resting microglia with the transplant may facilitate their role in antigen presentation under specific conditions of immune provocation.