A quantitative analysis of the microglial cell reaction in central primary sensory projection territories following peripheral nerve injury in the adult rat
- 1 September 1993
- journal article
- Published by Springer Nature in Experimental Brain Research
- Vol. 96 (1) , 19-27
- https://doi.org/10.1007/bf00230435
Abstract
The time course of the microglial cell reaction in central nervous system primary sensory projection territories has been examined following peripheral nerve injury in the adult rat using qualitative and quantitative analysis of immunoreactivity with the monoclonal antibody OX-42, which recognises the complement receptor CR3. The regions examined included the gracile nucleus, the column of Clarke and the spinal cord dorsal horn (superficial and deep laminae separately) after unilateral sciatic nerve transection, and the spinal trigeminal nucleus following unilateral infraorbital nerve transection. In all territories examined a qualitative increase in OX-42 immunoreactivity was observed 24 h postlesion. Further, quantitative analysis revealed an exponential development of the OX-42 immunoreactivity, with a peak at one week postlesion, thereafter showing a slow exponential decline. Our results show that the signal (or signals) that induces the microglial cell response in primary sensory projection territories is rapid in comparison to previously described central degenerative changes following peripheral nerve lesions (transganglionic degeneration). These findings are compatible with the hypothesis that activated microglia play a pathogenetic role in the development of transganglionic degeneration.Keywords
This publication has 36 references indexed in Scilit:
- Characterisation of two new monoclonal antibodies directed against rat microgliaJournal of Comparative Neurology, 1991
- Central projections of the sciatic, saphenous, median, and ulnar nerves of the rat demonstrated by transganglionic transport of choleragenoid‐HRP (B‐HRP) and wheat germ agglutinin‐HRP (WGA‐HRP)Journal of Comparative Neurology, 1991
- Ultrastructural changes of the central scalloped (C1) primary afferent endings of synaptic glomeruli in the substantia gelatinosa Rolandi of the rat after peripheral neurotomyJournal of Neurocytology, 1990
- Lectin binding by resting and reactive microgliaJournal of Neurocytology, 1987
- Transganglionic degeneration in vibrissae innervating primary sensory neurons of the rat: A light and electron microscopic studyJournal of Comparative Neurology, 1986
- Quantitative electron microscopic observations on the non‐neuronal cells and lipid droplets in the posterior funiculus of the cat after dorsal rhizotomyJournal of Comparative Neurology, 1985
- The cytoarchitectonic organization of the spinal cord in the rat. I. The lower thoracic and lumbosacral cordJournal of Comparative Neurology, 1984
- Somatotopic organization of the thoracic spinal nerve in the dorsal horn demonstrated with transganglionic degenerationJournal of Comparative Neurology, 1981
- Intraspinal non-neuronal cellular responses to peripheral nerve injuryThe Anatomical Record, 1979
- An ultrastructural study of transganglionic degeneration in the main sensory trigeminal nucleus of the ratJournal of Neurocytology, 1979