RELEASE AND RECONSTITUTION OF FC-RECEPTORS FROM NORMAL HUMAN MONONUCLEAR-CELLS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (1) , 47-52
Abstract
Nylon-fiber non-adherent cells isolated from human peripheral blood release their Fc receptors for Ig[immunoglobulin]G on culture in vitro in medium containing 0-2% fetal calf serum. The majority of Fc+ nylon-fiber non-adherent cells was reconstituted using supernates from different cell populations of human peripheral blood. The best reconstituting activity was detected in the supernates from glass-adherent cells, or unfractionated Lymphoprep cells, while those obtained from nylon-fiber non-adherent cells gave variable results sometimes containing no activity at all. Reconstitution was achieved by the uptake of Fc receptors present in the supernate which inhibited Fc rosette formation. Inhibition was also achieved with supernates from peripheral blood cells of a patient with monocytic leukemia. Only autologous but not allogeneic cell supernates could reconstitute Fc receptors. The reconstitution of Fc receptors on peripheral blood Fc-IgG lymphocytes by monocytic cells is probably under genetic control.