pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase
- 18 April 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 543 (1-3) , 37-41
- https://doi.org/10.1016/s0014-5793(03)00382-x
Abstract
Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl-adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl-adenylate to the acceptor nucleotideKeywords
This publication has 18 references indexed in Scilit:
- Identification of enzyme produced firefly oxyluciferin by reverse phase HPLCTetrahedron Letters, 2001
- Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap4A catalyzed by firefly luciferaseFEBS Letters, 1998
- Acyl-Adenylate Motif of the Acyl-Adenylate/Thioester-Forming Enzyme Superfamily: A Site-Directed Mutagenesis Study with the Pseudomonas sp. Strain CBS3 4-Chlorobenzoate:Coenzyme A LigaseBiochemistry, 1997
- Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent ReactionBiochemical and Biophysical Research Communications, 1997
- THE CHEMICAL MECHANISM and EVOLUTIONARY DEVELOPMENT OF BEETLE BIOLUMINESCENCEPhotochemistry and Photobiology, 1995
- Lanterns of the Firefly Photinus pyralis Contain Abundant Diadenosine 5′,5“′-P1,P4-Tetraphosphate Pyrophosphohydrolase ActivityPublished by Elsevier ,1995
- Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferaseEuropean Journal of Biochemistry, 1991
- Firefly luciferase synthesizes P1,P4‐bis(5'‐adenosyl)tetraphosphate (Ap4A) and other dinucleoside polyphosphatesFEBS Letters, 1990
- The Structure and Synthesis of Firefly LuciferinJournal of the American Chemical Society, 1963
- Spectral emission and quantum yield of firefly bioluminescenceArchives of Biochemistry and Biophysics, 1960