Artifact Produced in Disc Electrophoresis by Ammonium Persulfate
- 14 April 1967
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 156 (3772) , 256-257
- https://doi.org/10.1126/science.156.3772.256
Abstract
Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.Keywords
This publication has 2 references indexed in Scilit:
- An apparatus for preparative temperature-regulated polyacrylamide gel electrophoresisAnalytical Biochemistry, 1964
- The Reversible Denaturation Processes of Yeast Enolase*Biochemistry, 1964