Reaction of Halodeoxycellulose Gel Beads with Polymethylenediamines and Enzyme Immobilization onto Aminated Beads.
- 1 January 1994
- journal article
- Published by Society of Fiber Science and Technology Japan in Sen'i Gakkaishi
- Vol. 50 (11) , 520-525
- https://doi.org/10.2115/fiber.50.11_520
Abstract
Halodeoxycellulose gel beads were treated with polymethylenediamines under alkaline conditions in water or in dimethyl sulfoxide. Bromine atoms in bromodeoxycellulose gel beads were substituted with amino groups of diamines to yield cellulose derivatives having pendent amino groups, while the substitution hardly occurred for chlorodeoxycellulose samples. The aminated gel beads were treated with glutaraldehyde for immobilization of alkaline phosphatase. Azomethine bonds formed were reduced to stabilize the bonding between the enzyme and the support. Temperature and pH optima of the enzyme were not changed much by immobilization, while the value of apparent Michaelis constant increased. The length of diamine chain showed no influence on the apparent Michaelis constant and maximum velocity of the immobilized alkaline phosphatase within the number of methylene groups between 2 and 12.Keywords
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