High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells
- 15 September 1999
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 342 (3) , 497-501
- https://doi.org/10.1042/0264-6021:3420497
Abstract
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.Keywords
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