Protein synthesis of the sponge Geodia cydonium: Characterization of the system
Open Access
- 1 June 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (6) , 835-847
- https://doi.org/10.1093/nar/1.6.835
Abstract
The ribosomal population of the sponge Geodia cydonium has been examined. The monosomes have a sedimentation constant of 80 S, the sizes of the subunits are approximately 60 S and 45 S respectively. The polyribosomes contain up to 40 ribosomal units. Cell free protein synthesizing systems (cell homogenate as well as reconstituted system) have been prepared and characterized with respect to Mg2+, KCl and ATP concentrations, temperature, pH and time course of the reaction. In the cell-free system and in the cellular system the protein biosynthesis is inhibited by chloramphenicol. It is not affected by cycloheximide.Keywords
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