β-Galactosidase-Linked Immunoassay of Prostaglandin F2α1

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Abstract
A sensitive and specific enzyme immunoassay for prostaglandin F2α was developed in which prostaglandin F2α was labeled with β-D-galactosidase. After competitive binding to antibody between enzyme-labeled and free prostaglandin F2α, the immu-noreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined fluorometrically. The procedures allowed determination of 0.03–20 pmol of prostaglandin F2α- The detection limit of 0.03 pmol was comparable with that of previously reported radioimmunoassays. Enzyme immunoassay and radioimmunoassay showed almost the same cross-reactivity with other prostaglandins and metabolites.