Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria
Open Access
- 1 February 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (2) , 1015-1019
- https://doi.org/10.1128/aem.67.2.1015-1019.2001
Abstract
The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nreminitransposons in members of the α, β, and γ subclasses of theProteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.Keywords
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