Capillary Electrophoresis Micro X-ray Fluorescence: A Tool for Benchtop Elemental Analysis
- 3 April 2003
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 75 (9) , 2048-2053
- https://doi.org/10.1021/ac0207269
Abstract
A new tool was developed for separation and elemental detection by interfacing a simple capillary electrophoresis (CE) apparatus, constructed using a thin-walled fused-silica capillary, with a benchtop energy-dispersive micro X-ray fluorescence (MXRF) system. X-ray excitation and detection of the separated analytes was done using an EDAX Eagle II micro X-ray fluorescence system equipped with a polycapillary Rh target excitation source and a SiLi detector. It was demonstrated that this prototype system could be used for the separation and detection of species containing two different metals from one another, specifically Cu and Co. Free Co could also be separated from Co bound to cyanocobalamin (vitamin B-12). Two organic compounds were also separated from one another, a large biological protein, ferritin, from a small biological organic, cyanocobalamin. Preliminary average detection limits obtained on this system were on the order of 10-4 M and compared favorably to those reported for the similar technique of CE-synchrotron XRF. CEMXRF allows for nondestructive, simultaneous, on-line, benchtop elemental analysis for chemical speciation applications.Keywords
This publication has 11 references indexed in Scilit:
- Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)Electrophoresis, 2001
- Inorganic biological analysis by capillary electrophoresisThe Analyst, 2001
- Characterizing speciation of trace elements in the chemistry of life.Analytical and Bioanalytical Chemistry, 2001
- Recent progress in capillary electrophoresis of metal ionsElectrophoresis, 2000
- Capillary electrophoresis inductively coupled plasma mass spectrometryMicrochemical Journal, 2000
- On-line X-ray fluorescence detection for capillary electrophoresis separationsNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1999
- HighlightCapillary electrophoresis coupled to electrochemical detection: a review of recent advancesAnalytical Communications, 1998
- Characterization of metal complexes of pharmaceutical interest by capillary electrophoresis with element sensitive detectionJournal of Chromatography A, 1997
- Element-sensitive X-ray detection for capillary electrophoresisJournal of Chromatography A, 1996
- On-line coupling of PIXE with capillary zone electrophoresis (CZE)Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1996