Analysis of the serum antibody responses to type 1 and type 2 fimbriae in mice immunized with Actinomyces viscosus T14V

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Abstract
The antibody responses of inbred mice immunized with Actinomyces viscosus T14V cells were analyzed using enzyme‐linked immunoabsorbent assays (ELISAs) for measuring serum antibodies reactive with A. viscosus Tl4V cells and type 1 and type 2 fImbriae. In A/I mice immunized intraperitoneally on days 0 and 14, the serum antibody responses approached peak levels during d 19–35, and a dose of 108 cells/injection elicited optimal responses. Analysis of the responses of three genetically diverse strains of inbred mice indicated striking variations in the amounts of anti‐type 1 (6.5‐fold) and anti‐type 2 (14.3‐fold) antibodies elicited. The observed variations in the magnitude of the anti‐fImbrial antibody responses are theoretically of sufficient magnitude to account for signifIcant differences between mouse strains in their ability to inhibit adherence of A. viscosus T14V to saliva‐coated hydroxyapatite and other bacteria. These studies provide a model with which the effects of variations in anti‐fimbrial antibody responses on bacterial adherence may be analyzed.