Rapid Determination of Butylated Hydroxyanisole, tert-Butylhydroquinone, and Propyl Gallate in Edible Oils by Electron Capture Gas-Liquid Chromatography

1 November 1976

journal article
research article
Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL

Vol. 59 (6) , 1208-1212
https://doi.org/10.1093/jaoac/59.6.1208

Abstract

A rapid method is described for the determination of 2- and 3-tert-butyl-4-hydroxyanisole (2- and 3-BHA), tert-butylhydroquinone (TBHQ), and propyl gallate (PG) as their heptafluorobutyrates in edible oils. A solution of the oil in ether-benzene is treated with heptafluorobutyric anhydride (HFBA) in the presence of trimethylamine as catalyst. Excess HFBA is destroyed and a suitably diluted aliquot is analyzed by electron capture gas-liquid chromatography. An internal standard is added before derivatization to aid quantitation and identification of the antioxidants. Average recoveries from an oil spiked at 1, 5, 5, and 5 ppm TBHQ, 2-BHA, 3-BHA, and PG were 110, 105, 106, and 102%, respectively. At these low levels, however, some late-eluting peaks interfered. Butylated hydroxytoluene is not determined by this method.

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