A Review of Fluorescence in Situ Hybridization (FISH): Current Status and Future Prospects
- 1 January 2000
- journal article
- review article
- Published by Taylor & Francis in Biotechnic & Histochemistry
- Vol. 75 (2) , 54-78
- https://doi.org/10.3109/10520290009064150
Abstract
Fluorescence in situ hybridization (FISH) is a powerful technique for detecting DNA or RNA sequences in cells, tissues and tumors. This molecular cytogenetic technique enables the localization of specific DNA sequences within interphase chromatin and metaphase chromosomes and the identification of both structural and numerical chromosome changes. FISH is quickly becoming one of the most extensively used cytochemical staining techniques owing to its sensitivity and versatility, and with the improvement of current technology and cost effectiveness, its use will surely continue to expand. Here we review the wide variety of current applications and future prospects of FISH technology.Keywords
This publication has 151 references indexed in Scilit:
- Spatial Distributions of Early and Late Replicating Chromatin in Interphase Chromosome TerritoriesExperimental Cell Research, 1998
- Aneuploidy in late‐step spermatids of mice detected by two‐chromosome fluorescence in situ hybridizationMolecular Reproduction and Development, 1995
- High resolution mapping of overlapping cosmids by fluorescence in situ hybridizationCytometry, 1994
- Donor-specific bone marrow infusion after orthotopic liver transplantationThe Lancet, 1994
- A Fluorescence in Situ Hybridization Map of Human Chromosome 21 Consisting of 30 Genetic and Physical Markers on the Chromosome: Localization of 137 Additional YAC and Cosmid Clones with Respect to This MapGenomics, 1993
- High-Resolution Cytogenetic Mapping of 342 New Cosmid Markers Including 43 RFLP Markers on Human Chromosome 17 by Fluorescence in Situ HybridizationGenomics, 1993
- Waardenburg syndrome type I in a child with deletion (2) (q35q36.2)American Journal of Medical Genetics, 1992
- Isolation of 1001 new markers from human chromosome 11, excluding the region of 11p13–p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybridsGenomics, 1992
- A cytogenetic follow-up study of the victims of a radiation accident in Goiania (Brazil)Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1991
- The arrangement of interphase chromosomes: Structural and functional aspectsExperimental Cell Research, 1989