STUDIES ON PAPANICOLAOU STAINING .1. VISIBLE-LIGHT SPECTRA OF STAINED CERVICAL CELLS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 1  (3) , 160-168
Abstract
This paper presents visible-light absorbance spectra of the nuclei and cytoplasms of 10 types of cervical cells stained by the Papanicolaou technique, together with tables summarizing the data. For automated image processing of Papanicolaou smears the most suitable wavelengths are 531 nm to maximize the contrast of cell against background and a wavelength in the range 560-605 nm (with a mean at 575 nm) to maximize the contrast of the nucleus against the cytoplasm. [The Papanicolaou technique is used in cancer diagnosis].

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