Comparative immunological studies of mutarotases from mammals.

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Abstract
The antibodies against purified rat and hog kidney mutarotases (type II) were prepared by injecting each of the purified mutarotases into rabbits and quantitatively precipitated the enzyme activities of rat and hog kidney mutarotases, respectively. Immunological cross-reactivity among 4 forms of both rat and hog kidney mutarotases and among mutarotases from the kidney, liver and small intestine of 5 species of animals, rat, hog, mouse, beef and rabbit, was examined by using these antibodies. The 4 forms of mutarotase in rat kidney and those in hog kidney were immunologically identical. The kidney, liver and small intestine of each species evidently contain very similar enzymes, though there were clear differences between the enzymes from different species.

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