Measurement of Bovine Plasma Testosterone by Radioimmunoassay (RIA) and by a Rapid Competitive Protein Binding (CPB) Assay
- 1 October 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Animal Science
- Vol. 39 (4) , 720-724
- https://doi.org/10.2527/jas1974.394720x
Abstract
Changes in the peripheral plasma level of testosterone during the bovine estrous cycle were determined by a rapid competitive protein-binding assay and by radioimmunoassay, using a specific antiserum against testosterone following chromatographic purification of the steroid on Sephadex LH-20 columns. Values obtained by the two methods were similar. Testosterone concentrations increased during the 3 days preceding estrus and attained peak values (47 ± 9 pg/ml) 10 to 16 hr. before the onset of estrus. Minor rises were observed on days 3 to 5 and 11 to 14. The levels of testosterone observed during the cycle generally paralleled the plasma levels of estrogen observed in previous studies. Copyright © 1974. American Society of Animal Science. Copyright 1974 by American Society of Animal Science.Keywords
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