NITROGENOUS CONSTITUENTS OF BREWING MATERIALS: VI. USE OF ION-EXCHANGE RESINS IN FRACTIONATING THE NITROGEN COMPOUNDS OF BREWERS' WORTS

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Abstract
The recovery from ion-exchange resins of the nitrogenous compounds of wort has been found to be quantitative under selected conditions. It has further been found that, whereas the amino acids and certain simple peptides were adsorbed on cation exchange resins and were fractionally eluted in an unchanged condition by ammonia, the more complex nitrogenous compounds were partially degraded by these resins. The amino acids and simple peptides, prepared either by ion-exchange chromatography or by adsorption on carbon, were examined by paper chromatography when a number of compounds, as yet unidentified, were revealed in addition to the numerous identifiable amino acids, etc.