HUMAN BASOPHIL DE-GRANULATION IN DERMATOPHAGOIDES ALLERGIES - 93 CASES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 44  (5) , 308-312
Abstract
Basophil degranulation (HBD) caused by Dermatophagoides pteronyssinus (DP) and D. farinae (DF) allergens, often involved in house dust allergies were studied in 93 humans. The human basophil degranulation [HBD] technique was used. For each allergen, dilution ratios of 100.mu.g/ml, 10.mu.g/ml and I.mu.g/ml were used. The degranulation was significant (P < 0.001) for values > 35% (HBD positive). Of 30 subjects with a positive skin test with DP, 27 had a positive HBD; no HBD was obtained in subjects with negative skin test (95% concordance). With DF, the concordance level reached 86.6% in 30 subjects. Contrary to previous work conducted with grass pollen extracts, no significant correlation was found between the skin test diameter and HBD. This may be due to the variable allergenic potency of the Dermatophagoides extracts used for the skin tests. The HBD is confirmed as a simple and reliable technique for studying reaginic allergies.