Fat metabolism in higher plants. XXIV. A soluble beta-oxidative system from germinating seeds of Ricinus communis.

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Abstract
A soluble protein fraction obtained from endosperm of 5-day old germinated castor bean seeds contained both the enzymes from the [beta]-oxidation system and the glyoxylate bypass system. The mitochondrial fraction was a minor site for these activities. In the presence of ATP, CoA, Mn++, TPN, and DPN long chain fatty acids were readily degraded to acetyl CoA. Addition of ATP, Mn++, and glyoxylate to the system resulted in a rapid release of C14O2 from C14-carboxy labeled fatty acids. Evidence supports the concept that CO2 release is geared with the conversion of acetyl CoA and glyoxylate to CO2 and P-enolpyruvate via the glyoxylate bypass system rather than through a tricarboxylic acid cycle.