Nitric oxide‐releasing compounds inhibit Dictyostelium discoideum aggregation without altering cGMP production
- 7 December 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 314 (1) , 49-52
- https://doi.org/10.1016/0014-5793(92)81459-y
Abstract
The effects of nitric oxide‐releasing compounds on Dictyostelium discoideum cell development and guanylyl cyclase activity were studied. The addition of SNP (sodium nitroprusside) or SIN‐1 (3‐morpholino‐syndnonimine) to starved cells inhibited their differentiation and aggregation in a concentration‐dependent manner. In contrast to mammalian systems, SNP did not significantly affect guanylyl cyclase activity in cell lysates of D. discoideum nor did it stimulate cGMP production in intact cells. The results suggest that the inhibitory effects of NO on D. discoideum cell aggregation are through a mechanism independent of an effect on guanylyl cyclase activity.Keywords
This publication has 16 references indexed in Scilit:
- Cell-cell interactions in Dictyostelium developmentTrends in Genetics, 1989
- Cell fractionation, detergent sensitivity and solubilization of Dictyostelium adenylate cyclase and guanylate cyclaseMolecular and Cellular Biochemistry, 1987
- Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factorNature, 1987
- Streamers: Chemotactic Mutants of Dictyostelium discoideum with Altered Cyclic GMP MetabolismMicrobiology, 1981
- cAMP regulation of cell differentiation in Dictyostelium discoideum and the role of the cAMP receptorDevelopmental Biology, 1981
- Selective alterations in responsiveness of guanylate cyclase to activation by nitroso compounds during enzyme purificationBiochimica et Biophysica Acta (BBA) - General Subjects, 1981
- Guanylate cyclase activation in response to chemotactic stimulation in Dictyostelium discoideumFEBS Letters, 1978
- Guanylate Cyclase Activity in Dictyostelium discoideum and Its Increase during Cell DevelopmentDifferentiation, 1978
- Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cellsNature, 1975
- DYNAMICS OF ANTIGENIC MEMBRANE SITES RELATING TO CELL AGGREGATION IN DICTYOSTELIUM DISCOIDEUM The Journal of cell biology, 1973