Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies

Abstract
An enzyme-linked immunosorbent assay [ELISA] for the detection of antibodies [Ab] to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The ELISA can be specific for human immunoglobulin (Ig) M Ab when a .mu.-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue IgM Ab were demonstrated in a [human] infant suspected of having a recent dengue infection.

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