Abstract
A technique for the routine examination of biofilms using scanning electron microscopy was investigated. The resolution and magnification were better than that achieved by light microscopy and the high vacuum and coating procedures did not interfere with the biological structures. Scanning electron microscopy was used to examine the surfaces of support media for biological filtration. The surface of the plastic media was very flat compared to natural media but this did not inhibit bacterial adhesion. Fungi, bacteria and protozoans could be identified within the biofilm.

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