T-Cell Subpopulations in the Bone Marrow and Spleen from Thymectomized, Irradiated Mice Reconstituted with Normal or Nude Bone Marrow Cells

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Abstract
Adult thymectomized and lethally irradiated (ATxXB) BALB/c mice, reconstituted with nude or normal bone marrow (BM) cells, were studied to evaluate numbers and mitogen responsiveness of T cells and T cell progenitors in the BM and the spleen. Although mice reconstituted with nu/nu cells contained significantly higher numbers of Thy 1.2-positive cells in the BM than mice reconstituted with normal BM cells, smaller in vitro mitogen responses were generally given by the former mice. Spleen and BM cells of both groups responded to phytohemagglutinin and to concanavalin A. On the basis of comparisons with respones of BM and spleen cells from normal and Atx mice, anti-Thy 1.2 treatment of cells and 1 g velocity sedimentation experiments, most mitogen responders in the BM of ATxXB mice probably are Thy 1.2-negative, pre-thymic T cell precursors. These cells possibly are able to differentiate to some extent in the absence of the thymus.