GLYCOPROTEIN NATURE OF TANNASE IN ASPERGILLUS-NIGER

  • 1 January 1976
    • journal article
    • research article
    • Vol. 23  (4) , 293-298
Abstract
Tannase (tannin acyl-hydrolase, EC 3.1.1.20) was isolated from the culture medium of A. niger and purified about 200-fold. On polyacrylamide-gel electrophoresis it gave a single band. The MW of the enzyme was of the order of 55,000 as determined by gel filtration. The enzyme contains 21.5% of carbohydrates (mannose and glucose). Treatment of tannase with alkaline borohydride decreased the content of threonine, serine and mannose, suggesting that the carbohydrate-peptide linkage is of the O-glycoside type, involving mannose linked to threonine and serine.

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