The mechanism of internuclear transmission of SV40-induced complement fixation antigen in heterokaryocytes.

Abstract
Fusion of transformed human, monkey and hamster cells with cells of various origins results in the transmission of ICFA [induced complement fixation antigen] to the recipient nuclei. This transmission is insensitive to FUdR [5-fluoro-2[image]-deoxyuridine], indicating that DNA synthesis is not necessary. Actinomycin D inhibits ICFA transmission, indicating the necessity of new mRNA synthesis for transmission. Cycloheximide also inhibits the transmission of ICFA, indicating that protein synthesis is necessary for transmission; simple diffusion does not account for the observed internuclear protein migration.