“Matrix Effects” in Immunoassays
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 51 (sup205) , 105-112
- https://doi.org/10.3109/00365519109104608
Abstract
This article deals with “matrix effects” in immunoassay systems. As each component has its own matrix (buffer, antibody, separation agents), the term matrix effect - usually only referring to the matrix of the sample - has been replaced by the term interference effect. The possible sites of interference have been dealt with in turn, taking each component/step of an immunoassay separately. Results of assay improvement due to the removal of “matrix effects” are given using the PTH-assay as example. The change in results due to tampering with the serum matrix is given using the determination of free thyroxine in commercial quality control sera.Keywords
This publication has 40 references indexed in Scilit:
- Triton X-100 eliminates plasma proteins interference in a radioimmunoassay for luteinizing hormone-releasing hormone (LHRH) and LHRH analoguesJournal of Immunological Methods, 1989
- Interference of complement with the binding of carcinoembryonic antigen to solid-phase monoclonal antibodiesJournal of Immunological Methods, 1989
- Biochemical studies in peritoneal fluid from patients with acute pancreatitisDigestive Diseases and Sciences, 1987
- SAMPLE PRETREATMENT TO MINIMIZE INTERFERENCES FROM WHOLE BLOOD IN THE RADIOIMMUNOASSAY FOR CYCLOSPORINETransplantation, 1986
- A solid-phase enzyme immunoassay for C-reactive protein: Clinical value and the effect of rheumatoid factorJournal of Immunological Methods, 1984
- Use of inorganic salts to minimize serum interference in a sandwich enzyme immunoassay for human growth hormone using Fab'-horseradish peroxidase conjugateClinica Chimica Acta; International Journal of Clinical Chemistry, 1983
- THE MEASUREMENT OF SERUM THYROGLOBULIN IN THE PRESENCE OF THYROGLOBULIN ANTIBODIESClinical Endocrinology, 1981
- Interference by Polar Metabolites in a Direct Radioimmunoassay for Plasma AldosteroneAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1981
- 5 Congenital hypothyroidismClinics in Endocrinology and Metabolism, 1979
- Mechanism of interference by chelating agents and sucrose in radioimmunoassay of angiotensin IClinica Chimica Acta; International Journal of Clinical Chemistry, 1977