A COMPARISON OF CHEMICAL CHANGES IN PLANTS DURING COLD-HARDENING IN CONTROLLED AND NATURAL ENVIRONMENTS

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Abstract
Three cultivars of winter barley seedlings were subjected to six temperature regimes. Seedlings were collected at weekly intervals over the 4-week period and measured for chemical content and degree of hardening. The colder hardening temperatures resulted in lowering the killing point of the seedlings. Cultivar differences in killing temperature was slight. Except for the total soluble carbohydrates, the chemical changes in the three cultivars were not significantly different. The conditions of this study permitted the production of cold-hardened tissue in controlled environments similar to field-hardened tissue. The plants grown in the controlled environment increased in total soluble sugars to nearly the same extent as did plants grown in the field environment. Controlled environment-hardened plants increased in reducing sugars, total soluble nitrogen, and total nonsoluble residual nitrogen but not as much as did field-hardened plants. The controlled environment-hardened plants increased more in total lipids than did the field-hardened plants.

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