The Pulmonary Inflammatory Response to Experimental Fecal Peritonitis: Relative Roles of Tumor Necrosis Factor-α and Endotoxin

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Abstract
The roles of endotoxin (LPS) and tumor necrosis factor-α (TNF-α) in the causation of organ injury during sepsis are unclear. To study LPS and TNF-α in the genesis of lung inflammation after cecal ligation and puncture (CLP), we used endotoxin-resistant (C3H/HeJ) and endotoxin-sensitive mice (C3H/HeOuJ). We examined lung neutrophil sequestration, interleukin 1 (IL-1)β mRNA expression, IL-1β protein expression, and injury. We also determined the expression of two C-X-C chemokine mRNAs, macrophage inflammatory protein-2 (MIP-2) and KC, in the lung to determine whether in vivo, endotoxin, or TNF-α are significant modulators of MIP-2 and KC mRNA expression. After CLP, increased neutrophils sequestrated in the lungs of both strains of mice and coincided with an increase in expression of IL-1β, MIP-2 and KC mRNAs, and IL-1β protein. Lung and serum TNF-α were significantly increased in the C3H/HeOuJ strain but not in the C3H/HeJ strain. Histologic studies of the lung revealed similar injury in both strains. Our results suggest that bacterial factors other than endotoxin cause lung neutrophil sequestration and injury after CLP and further, that TNF-α production is not a prerequisite. Our findings also suggest a potential role for local pulmonary chemokine production in the control of neutrophil sequestration after CLP.