The effects of detergents on autolysis of 131-I-labelled rat thyroid homogenates

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Abstract
The effects of some detergents and of urea on autolysis of I-131-labelled whole rat thyroid homogenates have been investigated. Sodium dodecyl sulphate and urea inactivated the proteolytic enzymes. Do-decyltrimethylammonium chloride stimulated autolysis of homogenates at all times of labelling between 10 min. and 18 hr. The effect was most prolonged with the homogenate labelled for 18 hr. and most transient with the homogenate labelled for 30 min. Triton X-100 stimulated the autolysis of homogenates labelled for 2 hr. or 18 hr., but not that of homogenates labelled for 10 min. or 30 min. It had no effect on hydrolysis of particulate iodoprotein. Hydrolysis of I131-labelled particulate iodoprotein by thyroid homogenates has been studied. There was no difference in the rate of disappearance of labelled iodoprotein, whatever the time of labelling. Dodecyltri-methylammonium chloride stimulated hydrolysis of all the preparations of particulate iodoprotein to the same extent.