Effect of purified somatomedins on thymidine incorporation into lectin-activated human lymphocytes

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Abstract
The effects are described of purified preparations of several growth factors on thymidine incorporation into phytohemagglutinin-activated (PHA) human lymphocytes. The somatomedins selected for study included human stomatomedins A and C, insulin-like growth factors (IGF1 and IGF2) and multiplication-stimulating activity (MSA). Assays were carried out with and without serum. Complementary assays were performed with a low-molecular serum ultrafiltrate added to stomatomedin C and to MSA. All the peptides tested, except MSA, stimulated thymidine incorporation into PHA-activated lymphocytes in a dose-dependent manner, even though different doses were required to obtain a response. The data reported point out the multiplicity and the interrelationships of the serum factors involved in the stimulation of human cell growth.

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