Characterization of an Early Activation‐Dependent Antigen on Lymphocytes Defined by the Monoclonal Antibody BL‐Ac(F2)

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Abstract
A novel activation‐dependent lymphocyte cell‐surface antigen which is recognized by a MoAb, BL Ac(F2), is described. Although not found on resting lymphocytes the antigen is induced rapidly wiihin 2–4 h following stimulation of the cells using mitogens or antibodies against the T‐eell CD3 antigen and sIgM on B cells, respectively. Immunopreeipitation and Western Blotting indicated that the MoAb recognizes a molecule in a range of 78 85 kDa. Beyond its activalion‐dependent expression on lymphocytes the antigen was detected also on myelo‐monocytic cells. Expression kinetics and cellular distribution of this molecule suggest that it is distinct from previously described activation‐dependent cell‐surface antigens sueh as CD69, CD25 and 4F2.