ANION-EXCHANGE CHROMATOGRAPHY OF INOSITOL PHOSPHATES FROM SOIL

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Abstract
Extracts of 4 soils were passed through chromatographic systems. Some components of the extracts of each soil were eluted in well-defined zones simultaneously with inositol penta- and hexaphosphates. Another component, not found in mixtures of Na phytate and derivatives prepared in the laboratory, was eluted subsequently to the hexaphosphate. Inositol quadriphosphates were not demonstrated in soil. It was shown that these compounds could withstand the extraction procedure employed, although the mono-, di-, and triphosphates of inositol could not. 2/3 or more of the organic-P-containing material from soil that heretofore has been considered as phytin was shown to be admixed material that chemically behaves as phytin but that chromatographically is not the inositol shexaphosphate. When extracts of soils that had been incubated for 30 days with radioactive P were mixed with Na phytate and derivatives and passed through chromatographic systems, the curve of activity in the eluate followed the P31 curve over that portion of the run during which the 5 and 6 phosphate esters were being eluted. The incorporation of radioactive P into material inseparable from inositol hexaphosphate suggested a microbial synthesis of phytin in soil.

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