In-vitro development of zygotes from superovulated prepubertal and mature gilts

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Abstract
Summary. Ten prepubertal and 8 mature gilts were superovulated with PMSG and hCG, and inseminated with fresh boar semen. Zygotes were surgically recovered from oviducts 54–60 h after hCG. One and 2-cell zygotes were randomly allotted to Medium PL (modified BMOC-3 supplemented with 0·1 mm-EDTA and 1·5% BSA) or Medium G (Medium PL without pyruvate or lactate). Eggs were washed twice in medium, and placed in microdrops of medium overlaid with silicon oil for culture in an humidified 5% CO2, 5% O2, 90% N2 environment, then observed daily for 6 days. Development of eggs was dependent (P < 0·001) on the interactive effects of age of gilt (prepubertal versus mature) and medium type (PL versus G) used in culture. A greater proportion of eggs cultured in Medium G developed further than did eggs in Medium PL (P < 0·001). Additionally, a greater proportion of eggs from mature gilts developed further than did eggs from prepubertal gilts (P < 0·02). We suggest that these results provide evidence that zygotes resulting from superovulation regimens of prepubertal gilts do not possess the same capacity for in-vitro development as do zygotes from pubertal gilts. Keywords: zygote; blastocyst; prepubertal; pigs

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