Induction and Quantification of Microsclerotia inRosellinia necatrix

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Abstract
A new method was developed for the quantification of microsclerotia produced by R. necatrix [isolated from infected roots of apple trees]. The method, which is based on extraction of melanin from microsclerotia, provided a good correlation between the numbers of microsclerotia and the amount of extractable melanin. Red, blue and fluorescent (daylight-type) illumination induced microsclerotium formation, whereas near-UV light and darkness depressed the morphogenetic process. Scanning electron micrographs revealed that the microsclerotia were undifferentiated, irregular and rough; being composed of interwoven hyphae. [R. necatrix Prill. (imperfect stage Dematophora necatrix Hartig) causes severe damage to various plant and fruit trees, especially apple trees.].

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