Enumeration of VSV Particles and a Demonstration of Their Growth Kinetics by Electron Microscopy.

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Abstract
It is felt that a physical virus particle count coupled with a biological titer is a more useful method of viral quantitation than just the biological titer. Toward this end a particle count technique has been adapted to vesicular stomatitis virus. The agar sedimentation count procedure has demonstrated highly reproducible results. This technique has been used to study the growth kinetics of VSV in tissue culture using Earle''s L-cells. Growth curve studies indicate that the moi [multiplicity of infection] plays a major role in the replication and resultant quality of this virus. Consistent high yields can be obtained when the moi is kept at approximately 1. Natural aggregation studies using the agar sedimentation technique indicate that the virus is extruded from the cells in loosely connected chains. There are indications that the is a 11 ratio between particle count and biological titer.

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