Sulphate transport inCandida utilis
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 28 (1) , 6-11
- https://doi.org/10.1007/bf02877377
Abstract
Sulphate uptake byCandida utilis follows Michaelis-Menten type kinetics characterized by a Km of 1.43 mM for sulphate. The process is unidirectional, pH, temperature and energy dependent. Molybdate, selenate, thiosulphate, chromate and sulphite are competitive inhibitors. Dithionite is a mixed-type inhibitor of sulphate uptake. If cells are pre-incubated with sulphate, sulphite, thiosulphate, dithionite or sulphide, sulphate uptake is severely blocked. Inhibition by endogenous sulphate, sulphite and thiosulphate was specific for sulphate uptake. Thus, incorporation of extracellular sulphate seems to be under the control of a heterogeneous pool of sulphur compounds. These results are discussed in connection with the regulation of sulphur ammo acid biosynthesis inC.utilis.Keywords
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