cDNA‐directed expression of human thyroid peroxidase

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Abstract
A human thyroid peroxidase cDNA, hTPO‐1 [(1987) Proc. Natl. Acad. Sci. USA 84, 5555–5559], was expressed in human Hep G2 cells using a vaccinia virus cDNA‐expression system. When examined by immunoblot analysis, the level of hTPO‐1 protein expression reached a maximum approx. 24 h after infection and remained at a similar level up to 72 h post‐infection. The expressed protein was enzymatically active as measured by guaiacol oxidation. Monoclonal antibody‐assisted immunoaffinity column chromatography was used for partial purification of vaccinia‐expressed hTPO‐1, resulting in more than 300‐fold higher specific activity and a measurable difference spectrum of the hTPO‐1 (Fe3+)‐CN complex.