Effect of Cortisol on Nuclear RNA-Synthesis in vitro

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Abstract
Summary Nuclei isolated from rat liver cells can incorporate H3-cytidine triphosphate into RNA in vitro. Cortisol added to the incubation medium is taken up by the nuclei in small amounts, but does not stimulate nuclear RNA synthesis. Administration of the hormone in vivo, however, results in a marked increase in incorporation of H3-cytidine triphosphate incubated in vitro with nuclei isolated from 20 to 180 minutes after the injection. Synthesis of RNA in vitro is accompanied by a simultaneous partial degradation of RNA which affects the yields of RNA ex-tractable from nuclei even after very short periods of incubation.

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