Endocytosis of α1‐acid glycoprotein variants by human monocytic lineage cells
- 1 January 1993
- journal article
- research article
- Published by Wiley in Biology of the Cell
- Vol. 77 (2) , 187-193
- https://doi.org/10.1016/s0248-4900(05)80187-x
Abstract
Summary— Human α1‐acid glycoprotein (AGP or orosomucoid) is a major glycoprotein of plasma. AGP can be separated on immobilized concanavalin A into three variants bearing none (AGP A), one (AGP B) or two (AGP C) biantennary glycans. In this paper, we show, using flow cytometry and confocal microscopy, that AGP C which is eluted from concanavalin A with mannose, binds to human monocytes, monocyte‐derived macrophages as well as human promonocytic cell lines such as THP1 or U937. Conversely HL60, a promyelocytic cell line, does not express the surface AGP C binding protein. AGP C is internalized and degraded with an efficiency depending on the state of differentiation of these cells. In contrast, AGP A which is not recognized by concanavalin A, does not bind to any of these cells.Keywords
This publication has 0 references indexed in Scilit: