The human carcinoma cell line A431 possesses large numbers of functional β‐adrenergic receptors
- 24 April 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 169 (2) , 151-155
- https://doi.org/10.1016/0014-5793(84)80308-7
Abstract
The existence of β-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[3H]dihydroalprenolol and the hydrophilic antagonist [3H]CGP-12,177 as β-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the β2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human β-adrenergic receptors.Keywords
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