Imaging of the Surface Structures of Epon Thin Sections Created with a Glass Knife and a Diamond Knife by the Atomic Force Microscope
- 1 April 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Electron Microscopy
- Vol. 42 (2) , 121-123
- https://doi.org/10.1093/oxfordjournals.jmicro.a051012
Abstract
The surface of Epon thin sections of bacterial cells were imaged with an atomic force microscope. The sectioned surface created by a diamond knife was found to be smoother than that made by a glass knife. The bacterial shape embedded in epoxy resin can be clearly seen on a diamond knife section but not on a glass knife section. The contour of the embedded bacterial cell was imaged as an engraved shape in the Epon matrix. The depth of the engraving was about 6 nm on average measured at the cell wall ridge in the diamond knife section and 30nm in the glass knife section.Keywords
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