Optimization of 6-aminopenicillanic acid (6-APA) production by using a new immobilized penicillin acylase
- 1 December 2000
- journal article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 32 (3) , 173-177
- https://doi.org/10.1042/ba20000042
Abstract
A new immobilized penicillin acylase (ECPVA) was obtained by covalent binding of penicillin acylase from Streptomyces lavendulae on Eupergit C. Enzymic hydrolysis of penicillin V catalysed by ECPVA was optimized using a 2(3) factorial design of experiments, and the selected parameters for this study were pH, temperature and substrate concentration. The immobilized enzyme showed an optimal pH value of 9.5-10.5, and an optimal temperature of 60 degrees C, whereas its soluble counterpart showed the same optimal pH value and a lower optimal temperature of 50 degrees C.Keywords
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