FACILE AND DEFINITIVE DETERMINATION OF HUMAN ADRIAMYCIN AND DAUNORUBICIN METABOLITES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (3) , 152-156
Abstract
Adriamycin [an antineoplastic drug] and 8 metabolites were separated and characterized with an ammonium formate/tetrahydrofuran solvent system on a .mu.Bondapak-Phenyl column. Daunorubicin [an antineoplastic drug] and 7 daunorubicin metabolites were separated and characterized by the same method. Adriamycin and its maor metabolite, adriamycinol, were extracted with 50-70% efficiency from human plasma with chloroform/2-propanol (1:1) and quantified against a daunorubicin internal standard. Urinary concentrations of parent drug, adriamycinol and [demethyldeoxyadriamycinol aglycone 4-O-sulfate] were determined. Daunorubicin and daunorubicinol were extracted from human plasma and quantified against an adriamycinol internal standard. The fluorescence detection system has a detection limit of 1.0 pmol; levels as low as 25 pmol/ml are measurable in plasma.

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