The sequence GGCmCGG is resistant toMspI cleavage
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (11) , 3559-3569
- https://doi.org/10.1093/nar/11.11.3559
Abstract
MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.Keywords
This publication has 14 references indexed in Scilit:
- A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene, 1982
- DNA methylation in chicken alpha-globin gene expression.Proceedings of the National Academy of Sciences, 1982
- The construction of cosmid libraries which can be used to transform eukaryotic cellsNucleic Acids Research, 1982
- The 3′ untranslated regions of the duplicated human α-globin genes are unexpectedly divergentCell, 1980
- Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fdGene, 1980
- Human fetal gγ- and Aγ-globin genes: Complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genesCell, 1980
- The chromosomal arrangement of human α-like globin genes: Sequence homology and α-globin gene deletionsCell, 1980
- DNA methylation in the human γδβ-globin locus in erythroid and nonerythroid tissuesCell, 1980
- A novel type of secondary modification of two CCGG residues in the human γδβ-globin gene locusNucleic Acids Research, 1980
- Use of restriction enzymes to study eukaryotic DNA methylationJournal of Molecular Biology, 1978