Increased Glycan Chain Length Distribution and Decreased Susceptibility to Moenomycin in a Vancomycin-Resistant Staphylococcus aureus Mutant
Open Access
- 1 January 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (1) , 75-81
- https://doi.org/10.1128/aac.46.1.75-81.2002
Abstract
A vancomycin-resistant Staphylococcus aureus mutant, COL-VR1 (MIC, 16 μg/ml), was isolated from methicillin-resistant S. aureus COL by exposure to vancomycin. COL-VR1 also showed decreased susceptibility to teicoplanin (8-fold), methicillin (2-fold), macarbomycin (8-fold), and moenomycin (16-fold). Macarbomycin and moenomycin are thought to directly inhibit transglycosylase activity. Characterization of the mutant revealed a thickened cell wall and suppression of penicillin-induced lysis, although the amounts of the five penicillin-binding proteins (PBPs 1, 2, 3, 4, and 2′) and the profiles of peptidoglycan hydrolases were not altered. Analysis of muropeptide profile and glycan chain length distribution by reversed-phase high-pressure liquid chromatography revealed slightly decreased peptide cross-linking and an increased average glycan chain length compared to those of the parent. These results together suggest that a transglycosylase activity was enhanced in the mutant. This may represent a novel mechanism of glycopeptide resistance in S. aureus .Keywords
This publication has 30 references indexed in Scilit:
- Clinical Isolate of Vancomycin-Heterointermediate Staphylococcus aureus Susceptible to Methicillin and In Vitro Selection of a Vancomycin-Resistant DerivativeAntimicrobial Agents and Chemotherapy, 2001
- A Spectrum of Changes Occurs in Peptidoglycan Composition of Glycopeptide-Intermediate Clinical Staphylococcus aureus IsolatesAntimicrobial Agents and Chemotherapy, 2001
- Involvement of multiple genetic loci inStaphylococcus aureusteicoplanin resistanceFEMS Microbiology Letters, 2001
- Characterization of Staphylococcus aureus Cell Wall Glycan Strands, Evidence for a New β-N-Acetylglucosaminidase ActivityPublished by Elsevier ,2000
- Triton X-100 alters the resistance level of methicillin-resistantStaphylococcus aureusto oxacillinFEMS Microbiology Letters, 1995
- The effect of Triton X-100 on the in-vitro susceptibility of methicillin-resistant Staphylococcus aureus to oxacillinJournal of Antimicrobial Chemotherapy, 1994
- Nucleotide sequence of the structural gene for the penicillin-binding protein 2 ofStaphylococcus aureusand the presence of a homologous gene in other staphylococciFEMS Microbiology Letters, 1994
- THE STRUCTURE AND MODE OF ACTION OF GLYCOPEPTIDE ANTIBIOTICS OF THE VANCOMYCIN GROUPAnnual Review of Microbiology, 1984
- Polymerization by transglycosylation in the biosynthesis of the peptidoglycan of Escherichia coli K 12 and its inhibition by antibioticsFEBS Letters, 1978
- Moenomycin, an inhibitor of cell wall synthesisBiochemical and Biophysical Research Communications, 1968