Investigations on the Mechanism of Induction of the Alkaline Phosphatase by Bromodesoxyuridine in Herpes simplex Virus Transformed Cells and the Transport of Uridine
Open Access
- 1 December 1980
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 35 (11-12) , 1036-1045
- https://doi.org/10.1515/znc-1980-11-1230
Abstract
Addition of BrdUrd [bromodeoxyuridine] in combination with prednisolone to HSV-[herpes simplex virus type 1]-transformed hamster embryo cells induces an alkaline phosphatase (AP. FdUrd enhances, dThd reduces the inducing capacity of BrdUrd and prednisolone. Induction is prevented by addition of cycloheximide or of cytosine arabinoside. BrdUrd reduces transport and phosphorylation of exogenously applied labeled Urd, especially the amount of UTP, UDP and Urd-diphosphate sugars. The Lineweaver-Burk-plot of uridine-uptake after addition of BrdUrd reveals the characteristics of the mixed type inhibition (competitive-noncompetitive).Keywords
This publication has 2 references indexed in Scilit:
- Regulation of alkaline phosphatase expression in human choriocarcinoma cell lines.Proceedings of the National Academy of Sciences, 1979
- INDUCTION OF ALKALINE-PHOSPHATASE ACTIVITY IN HELA-CELLS BY CHOLINE CHLORIDE1978