Abstract
When a housefly (Musca domestica L.) or the yellow‐fever mosquito (Aëdes aegypti L.) flies through a finely dispersed insecticidal mist a large proportion of the dose accumulated is found on the wings. If Sudan III is added to the insecticide the dye either penetrates into the wings directly and later appears in the malpighian tubules or it may be removed during the cleaning processes and absorbed through the legs.A colorimetric method of estimating the quantity of spray accumulated by flying insects is described, and it has been employed to investigate the effect which changes in the properties of the mist dispersions and the behaviour of the insects have on the quantity of spray accumulated. Briefly it may be said that within limits the dose accumulated increases with the activity of the insect and the size of the mist particle. The method has also been used to measure the surface median lethal doses of pyrethrins, 2:2‐bis (parachlorophenyl)‐1: 1:1‐trichlorethane (D.D.T.) and 1:2:3:4:5:6‐hexachlorocyclohexane (666) to Musca and Aides.