RADIOIMMUNOASSAY OF THYROTROPHIN RELEASING HORMONE (TRH)
- 1 November 1974
- journal article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 77 (3) , 417-421
- https://doi.org/10.1530/acta.0.0770417
Abstract
Immunization of rabbits with a conjugate in which L-pGlu-L-His-L-Pro-NH2 (TRH) at the amide side has been coupled to bovine serum albumin (BSA) yielded anti-TRH antibody which was detected by reaction of diluted antiserum with 3H-TRH. A double antibody radioimmunoassay has been developed in which 100 pg unlabelled TRH could be detected. Several analogues tested showed that only pGlu-His-Pro-OMe possessed comparable reactivity, while all the other compounds exhibited very little activity in the assay.Keywords
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