Über Hämochromogene

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Abstract
The formation of a hemochromogen, which consists in the addition of two mols of a N containing compound to a porphyrin-Fe-complex salt(heme), is considered to be a reversible reaction. The expts. descr. were undertaken to solve various problems relating to cytochrome c, which itself represents a hemochromogen system. Protein hemochromogen systems were most suitable for these studies, globin being the protein used. In view of the strongly selective absorption of the hemochromogen in the visible part of the spectrum, the spectrophotometric method of analysis was adopted. Formulas were developed for the spectrophotometric detn. of heme and hemochromogen when both were present in the soln. To measure the maximum combining power of globin with heme, constant amts. of globin were brought together with increasing amts. of heme, and the resulting hemochromogen conc. detd. When the globin conc. was between 10-4 and 10-5 mol., all the added meso- or protoheme was used in the formation of the hemochromogen up to a molecular ratio of globin to heme of 14. The protein compound of cytochrome c was able to bind 4 mols of heme with relatively great affinity, and at concs. of the components of 2 .10-5 mol., no dissociation of the proto-cytochrome-protein-hemochromogen occurred. The formation of mixed hemochromogens with cytochrome c was possible only in an alkaline reaction mixture. Detailed directions were given for the various exptl. procedures.

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